[关键词]
[摘要]
目的 采用高效毛细管电泳法 (HPCE)建立葛根芩连汤的指纹图谱。方法 电解质溶液由 30 mm ol/ L磷酸盐和 40 m mol/ L硼酸盐组成,未涂层融硅毛细管 (65 cm× 50μm,有效长度 43cm ),分离电压 22kV,进样 1s,检测波长254 nm,温度 30℃。结果 上述条件下,葛根芩连汤中27个成分得到了较好的分离,方法学考察结果符合定量测定和定性研究的要求。结论 该方法具有较好的分离效果和良好的精密度,可考虑作为葛根芩连制剂的质量控制方法
[Key word]
[Abstract]
Object To study the HPCE fingerprinting of Gegen Qinlian Decoction (GQD). Methods A buffer was composed of 30 mmol/L sodium phosphate and 40 mmol/L borate solution. Capillary electrophoresis was performed using a 65 cm (43 cm to detector) ×50 μm fused-silica capillary tube. Separation voltage was 22 kV, sampling time was 1 s, detected wavelength was 254 nm, and the temperature was maintained at 30 ℃. Results The 27 components in GQD were successfully separated. The observation of methodology was in keeping with quantitatine determination and qualitative study. Conclusion This method can be used for the quality control of the preparation of GQD with good precision.
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[基金项目]
国家自然科学基金重点资助项目(39970886);广东省自然科学基金资助项目(001070)