[关键词]
[摘要]
目的 建立液相色谱/蒸发光散射 (HPLC-ELSD)测定四物汤煎液中 D-果糖、D-葡萄糖和蔗糖含量的方法。方法 对四物汤煎液进行稀释、醇沉等处理后采用 HPLC-ELSD法分析。结果 1mL四物汤煎液中含 D-果糖(33.4±1.5)mg,D-葡萄糖 (24.2۪.9) mg,蔗糖 (112.7۰.1)mg;D-果糖在 0.15～3.75 mg/mL,D-葡萄糖和蔗糖在 0.15～5mg/mL,具有良好的线性关系 ;回收率为 D-果糖 128.5 %,D-葡萄糖 114.7%,蔗糖 124.7% ;日内RSD为 D-果糖 3.0 %,D-葡萄糖3.2 %,蔗糖4.4 %。结论 四物汤中含有丰富的单、双糖 ;HPLC-ELSD可用于四物汤中单、双糖类物质的分析。
[Key word]
[Abstract]
Object To establish a method by high performance liquid chromatography/evaporative light scattering detector (HPLC-ELSD) for the determination of contents of D-fructose, D-glucose, and sucr ose in Siwu Decoction ( SWD) . Methods Condensed SWD was analyzed by HPLC-ELSD after dilution,precipitation by ethanol. Results Condensed SWD, 1mL, contained ( 33.4۫.5) mg of D-fructose,( 24.2۪.9) mg of D-glucose and ( 112.7۰.1) mg of sucrose. The standard curves were linear within the range of 0.15—3.75 mg/mL for D-fructose and 0.15—5mg/ mL for D-glucose and sucrose.The recovery rates were 128. 5% for D-fructose, 114. 7% for D-glucose, and 124. 7% for sucrose. The relative standard deviations ( RSD) within-days were 3. 0% for D-fr uctose, 3. 2% for D-glucose, and 4. 4% for sucrose. Conclusion SWD contains abundant monosaccharide and disaccharide. HPLC-ELSD can be used to analyse the monosaccharide and disaccharide in SWD.
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[基金项目]
国家自然科学基金资助项目(30271617,30070913);军队“十五”重点项目(01Z019);北京市“二四八”重大创新工程项目(H010210220113)
