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[摘要]
目的 采用 HPLC/UV/ MS法对密花石斛进行指纹图谱研究。方法 采用 Agilent Zorbax SB- C1 8柱 ;甲醇和水为流动相进行梯度洗脱 ;流速为 1.0 mL/min。结果 得到分离度较好的密花石斛 HPL C/ U V指纹图谱,标示了9个共有峰,并用 HPL C/ MS对其部分色谱峰进行了初步定性。结论 该方法可用于密花石斛指纹图谱的测定,并为其全面质量控制提供参考。
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[Abstract]
Object To establish a method for studying the fingerprints of Dendrobium densiflorum Lindl. exWall . by HPLC/UV/MS. Methods Separation was performed on Agilent Zorbax SB-C18 column.Gradientelute was performed by the mobile phase consisting of methanol and water with the flow rate of 1.0 mL/min. Results Perfect fingerprints were obtained by HPLC/UV which can be used for the evaluation of D. densiflorum. Nine common peaks in the fingerprints were confirmed, and qualitative analysis of some other peaks were carried on by HPLC/MS. Conclusion The method can be applied to the quality control and studying on the chemical constituents of D.densiflorum crude drug.
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