目的 利用超临界 CO2 萃取结晶技术纯化穿心莲内酯。方法 采用系统观察法考察了超临界 CO2 萃取结晶分离对穿心莲内酯晶体形态、纯度、结晶量的影响,并采用扫描电镜、HPL C法分析结果。结果 穿心莲内酯晶体形态随压力的升高而变细、变短 ;随着压力的升高,晶体纯度提高,结晶量增加。结论 超临界 CO2 能萃取并同步高效结晶分离穿心莲内酯,为开发高质量中药活性成分提供一条捷径。

Object To separate and purify andrographolide by supercritical CO₂ extraction and crystallization. Methods The method of systematic observation was used to investigate the crystal appearance,purity and mass of andrographo lide by supercritical CO₂ extraction and crystallization, analyzing by scan-ning electron microscope (SEM ) and HPLC. Results The higher the pressure, the smaller and shorter the crystal shape, and the purer the purity, the more the mass of andrographolide. Conclusion This technique is a innovation of traditional supercritical fluid extraction (SCFE ) technique, which realizes highly effectual separation of andrographolide by supercritical CO₂ extraction and synchronous crystalliza-tion, developes a new technology to separate andrographolide and other active ingredients from Chinese traditional herbs.

国家自然科学基金资助项目 (29976008)