提出“中药成分组合效应”假说,即 :不管是单味中药还是中药复方,针对其主治证,均有与之对应的功效,其药效物质基础则是其中所含部分有效成分的最佳比例组合;并在脑缺血动物模型上进行初步验证。在毓神口服液的制剂工艺实验过程中用高效液相色谱与药理学实验相结合的方法,选择毓神口服液对脑缺血大鼠海马 CA1区的神经保护作用作为药效学评价指标,考察各样品的色谱图与药效学结果的相互关系,寻找能够体现药效的色谱峰。R1,R2,R3分别是与毓神口服液神经保护作用的药效密切相关的 3个峰,这 3个峰的相对比例而不是其绝对量对毓神口服液产生神经保护作用是非常关键的。该研究结果支持“中药成分组合效应”假说。

To propose the hypothesis of the requirement for optimum ratio of various active cons tituents of Chinese materia medica with desired effects, which suggests that both single drug and compound drug prescriptions have their own target effects corresponding to their main indications and their active constituents must be in optimum ratio This hypothesis was initially tested in an ischemic animal model The neuroprotection of Yushen Oral Liquor (YSOL, an oral liquor made of Chinese materia medica) on neuronal injury in CA1 region of rat hippocampus following transient forebrain ischemia was used as an index to evaluate the drug's effect By combination of HPLC and pharmacological method, the relationship of HPLC and pharmacodynamical Results of each sample made of YSOL was studied to observe the chromatogram peaks representing the drug's neuro protective effects There are three peaks named as R1, R2, and R3 for YSOL, which closely correlate to the drug's neuronal protective effects and the relative weight but not the absolute quantity of those peaks plays a critical role in producing neuronal protective effects of YSOL on ischemia induced neuronal damage The Results provide experimental data supporting our proposed hypothesis.

国家杰出青年基金(30125013);军队杰出青年基金(01J009);教育部“长江学者”奖励计划