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[摘要]
目的 通过长春花叶内生真菌的分离 ,筛选产生长春新碱的菌株。方法 从长春花叶中分离内生真菌 ,对其发酵提取物进行 TL C和 HPL C分析。结果 从长春花叶中分离筛选到一株无孢菌群菌株 :97CY3。它能产生长春新碱 ,HPL C测定其长春新碱含量约为 0 .2 0 5 μg/ L。结论 长春花内生真菌中 ,有的菌株可产生与宿主所产相同的抗癌物质长春新碱。
[Key word]
[Abstract]
Object To select the endophytic fungi which produce vincristine by isolating fungi from the leaves of Catharanthus roseus (L.) G. Don. Methods The endophytic fungi were isolated from the leaves of C. roseus and the zymotic extracts were analyzed by TLC and HPLC. Results An endophytic fungus which is Mycelia sterilia 97CY3 can produce vincristine. The content of vincristine in the fungus was determined as 0.205 μg/L by HPLC. Conclusion Some endophytic fungi isolated from C. roseus can produce the anticancer substance vincristine which is the same as that of host plant producing.
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[基金项目]
国家自然科学基金资助项目(39860005)