[关键词]
[摘要]
目的 评价和比较大青叶 5个化学部位的抗内毒素作用。方法 采用动态浊度法测定细菌内毒素浓度 ,观察大青叶不同化学部位对放线菌素 D敏化小鼠内毒素致死攻击的保护作用。并以内毒素制备家兔发热模型 ,测定其肛温变化。结果 大青叶 部位能直接中和降解内毒素 ,显著降低内毒素的致热性和致死性。结论 大青叶 部位具有显著的体内外抗内毒素活性。
[Key word]
[Abstract]
Object To evaluate and compare the antiendotoxin activity of five different fractions isolated from Folium Isatidis. Methods With kinetic turbidimetric assay,the bacterial endotoxin content of the testing samples was determined. The protection for the mice sensitized by actinomycin D from endotoxin-induced lethality was investigated. Changes of the rectal temperature were observed in feverous rabbits induced by endotoxin. Results The fraction Ⅳ from Folium Isatidis can neutralise endotoxin directly,and inhibit pyrogenicity and lethality induced by endotoxin. Conclusion The fraction Ⅳ from Folium Isatidis shows to have remarkable antiendotoxin activity in vitro and in vivo.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(39800193)