[关键词]
[摘要]
目的鉴定我国中草药丹参病毒病的病原及其种类。方法从自然感染引起花叶、矮化、褪绿和斑驳的丹参植株上分离病毒病原,进行生物学接种、电镜观察、双抗体夹心酶联免疫吸附测定、逆转录聚合酶链式反应及基因克隆和序列分析。结果通过指示植物症状、电镜下病毒粒体形状和血清学反应发现病毒分离物与黄瓜花叶病毒有较近的亲缘关系,初步将该病毒分离物鉴定为黄瓜花叶病毒。通过逆转录聚合酶链式反应及基因克隆和序列分析,证明该病毒分离物为一个黄瓜花叶病毒新株系。结论感染丹参的病毒病原为黄瓜花叶病毒,这是首次在我国中草药丹参上发现黄瓜花叶病毒。
[Key word]
[Abstract]
Object To identify the virus disease pathogens and its species from Danshen(Salvia miltorrhiza Bge)which is an important kind of Chinese traditional and herbal drugs in China Methods The virus pathogens were isolated from naturally infected Danshen with symptoms of mosaic, stunting, chlorosis and mottle, and the isolates were identified by biological inoculation, electron microscope observation, double antibody sandwich enzyme linked immunosorbent assay(DAS ELISA), reverse transcriptase polymerase chain reaction(RT PCR) detection and gene cloning and sequence analysis Results By the indicative plant symptoms, virus particles size in electron microscope and serological reactions, the virus isolate was found to be very similar to cucumber mosaic cucumovirus(CMV) and it was identified as CMV primarily RT PCR detection, gene cloning and sequence analysis demonstrated that the isolate was a new CMV isolate Conclusion The virus disease pathogen infecting Danshen was CMV, this is the first reporting of a new CMV isolate from Danshen in China.
[中图分类号]
[基金项目]
河北省科技攻关项目资助(03220111)