[关键词]
[摘要]
目的研究产地、生长季节及采后处理方式对杜仲皮、叶中5种主要生物活性成分含量的影响。方法利用HPLC法测定杜仲皮、叶中5种主要生物活性成分的含量。结果不同产地的杜仲皮、叶其生物活性成分含量相差悬殊,且同一产地的杜仲皮、叶中各种主要生物活性成分的含量没有一定相关性,并不随其他成分含量的增加而增加。杜仲中生物活性成分含量随季节变化而变化,杜仲皮、叶的最佳采收季节分别为4,5和7月,且采收后的杜仲皮、叶应及时处理使酶失活,避免自然干燥过程中胞内酶分解活性成分。此外,杜仲皮和叶中生物活性的含量差异很大,但有一定的互补性。结论产地、生长季节及采后处理方式对杜仲皮、叶中5种主要生物活性成分含量的影响很大,且不能简单的以叶代皮。
[Key word]
[Abstract]
Object To study the effects of districts, seasonal variations and treatments in past harvest on the five main bioactive components in the barks and leaves of Eucommia ulmoides Oliv Methods The contents of them in the bark and leaves of E ulmoides were determined by RP HPLC Results The contents of(+) pinoresinol di O β D glucopyranoside(PG),(+) syringaresinol di Oβ D glucopyranoside(SG), chorgenic acid(CGA), geniposide(GP), geniposidic acid(GA) among the sample of E ulmoides from different growing districts were obvious difference Besides, the amount of different bioactive components in the same sample had no correlation Seasonal variation and different past harvest treatments had great influence on the bioactive component in E ulmoide. Based on the monthly variation of their main bioactive component, the suitable seasons for harvest of the barks and leaves of E ulmoides are April, May and July respectively, and intracellular enzymes in the fresh barks and leaves collected should be inactivated imediately before drying to avoid lossing the bioactive components In addition, between the barks and leaves of E ulmoides, the major bioactive component had great differences, but had complementarity Conclusion The districts, seasonal variatons and past harvest treatments have great effects on the contents of five main bioactive components in the barks and leaves of E ulmooides, and the barks couldn't be replaced by the leaves simply.
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[基金项目]
湖北省重点攻关课题(952P0506)