[关键词]
[摘要]
目的 为了促进新疆紫草大面积人工栽培,缓解自然资源匮乏的现状,通过组织培养技术建立了新疆紫草的无性繁殖体系。方法 诱导新疆紫草多种外植体脱分化均已成功,胚状体扩增后萌发出大量的试管苗 ;愈伤组织扩增后诱导出大量的不定芽,不定芽经诱导生根后,得大量的试管苗,试管苗土栽成活。结果 外植体脱分化期间,2,4 - D不可缺少,KT的作用优于 BA;嫩芽切段脱分化最快,愈伤组织质量最好 ;降低 2,4 - D浓度有利于愈伤组织扩增和再分化 ;一定浓度 KT或 BA都可诱导出不定芽,前者还有利于球形胚的形成 ;球形胚培养初期,培养基中加入适量激素能使球形胚大量增殖,并很快发育 ;适当的昼夜温差 (10℃~ 2 8℃ )和自然光照有利于壮苗的形成和移栽成活。在移栽的 2 36株试管苗中,31株土培成活,成活率达 13.2 %。结论 通过组织培养方法建立的新疆紫草无性繁殖体系稳定、可用
[Key word]
[Abstract]
Object In order to promote the artificial cultivation of Arnebia euchroma (Royle) Johnst, and save the wild herb from relieving the lack of resources, the asexual multiplication system of A euchroma was established by tissue culture technique methods Dedifferentiation in many kinds of explants of A euchroma by inducement succeeded After the embryos were multiplied, there were a lot of germinated transplants in media After callus were multiplied, many adventitious buds had formed in the media, then they were rooting; the transplants formed by inducement and the plants survived after being cultured in soil Results During the dedifferentiation, 2,4 D was essential, the effect of KT was better than BA The shoot sections were dedifferentiated in the most rapid way among the explants The callus from them were in very good quality It is of benefit to the callus multiplication and redifferentiation after reducing the concentration of 2,4 D Both KT and BA can induce adventitious buds, KT was also suitable for the formation of globular shaped embryos In initial period of sphere embryos culture, it was necessary to add the plant growth regulators in media for multiplying sphere embryos and promoting their development Proper temperature difference between day and night (10 ℃-28 ℃) and natural light were helpful for the formation of strong sprouts and survival of transplants The survival rate was 13 2% with 31 plantlets survived among 236 transplants cultured in soil Conclusion The asexual multiplication system of A euchroma established through tissue culture is stable and available
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[基金项目]
国家自然科学基金资助项目(38960025)