目的 通过对葛根芩连汤 (GQD)各配伍组合对肺炎链球菌的抑菌作用差异来探讨其配伍规律。方法 采用液体试管两倍稀释法测定各煎液对肺炎链球菌的最低抑菌浓度 (MIC),比较各配伍组合后的药效学改变。结果各配伍煎液的 MIC不全相同。结论 GQD各配伍组合水煎液对肺炎链球菌抑菌作用不全相同 ;在全方中,抑菌作用药物主要为黄连与黄芩,炙甘草与葛根无明显抑菌作用 ;两药与黄芩、黄连配伍时对其抑菌拮抗作用不同

Object To explore the regularity of recipe composition by observing different bacteriostasis among compositions of Gegen Qinlian Decoction (GQD) against Streptococcus pneumoniae. methodsTo evaluate the minimal inhibitory concentration (MIC) of 15 decoctions through double fold dilution method. Results Not all of the decoctions are of the same MIC. Conclusion Not all of the 15 decoctions have the same bacteriostasis against S. pneumoniae. Among the four herbs of GQD Coptis chinensis Franch. has the strongest bacteriostasis and the next Scutellaria baicalensis Georgi. While Glycyrrhina wralensis Fisch and Pueraria lobata (Willd.) Ohwi have no bacteriostasis, but they can influence the bacteriostasis of C. chinensis and S. baicalensis when combinating with them.

国家自然科学基金资助项目(39970886);广东省自然科学基金项目(001070);军队“十五”计划基金重点课题(01z053)