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[摘要]
目的测定不同产地、不同部位红花五味子的五味子酯甲(schisantherinA)、去氧五味子素(五味子甲素,deoxyschizandrin)、五味子乙素(schisandrinB)和五味子丙素(schisandrinC)含量。方法HPLC法,使用Sphere-cloneODS柱;流动相:水(A),甲醇(B),梯度洗脱:0~4min,70%B;4~54min,70%~100%B;流速为0.4mL/min;柱温:25℃;检测波长:254nm。结果五味子酯甲、甲素、乙素、丙素在红花五味子中含量分别为0.026%~0.083%,0.007%~0.945%,0.002%~0.121%,0.010%~0.038%,其中五味子甲素普遍存在,且果实中含量高于茎藤。结论去氧五味子素是红花五味子果实的主要活性成分。
[Key word]
[Abstract]
Object To determine the contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in the stems and fruits of Schisandra rudriflora (Franch ) Rehd. et Wils, which were collected from different areas.Methods An HPLC method was set up: Column, Sphereclone, ODS (250 mm×4 6 mm, 5 μm); mobil phase, A: H2O, B: MeOH; gradient elution was with 70% B from 0-4 min, 70%-100% B from 4-54 min; the flow rate was 0 4 mL/min; the column temperature was 25 ℃ and the DAD detector was used at 254 nm.Results The contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in S. rubriflora were 0.026%-0.083%, 0.007%-0.945%, 0.002%-0.121%, 0.010%-0.038%, respectively. Deoxyschizandrin exists widely in S. rubriflora and its content is higher in fruits than that in stems. Conclusion Deoxyschizandrin is the main active lignan in the fruits of S rubriflora
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[基金项目]
高校博士点基金项目(200026519);国家自然科学基金项目(30271586);高校优秀青年教师教学和科研奖励基金项目(1999-71)