目的观察川乌配伍防己对动物免疫功能的影响,探讨寒热配伍的免疫作用。方法采用免疫变态反应性炎症模型及小鼠迟发性超敏反应(DTH)模型,并检测小鼠单核巨噬细胞(M^Φ)吞噬功能和特异性抗体生成等。结果川乌配伍防己既可明显抑制免疫增高小鼠DTH,明显抑制大鼠佐剂继发性关节炎,又明显提高免疫低下小鼠M^Φ吞噬功能。而各单味药则无明显作用。另外,对特异性体液免疫的影响,单味防己有明显促进作用,而乌防配伍后能明显抑制其作用。结论川乌配伍防己对细胞免疫有双向调节作用,对体液免疫也有一定的调节作用。

Object The immunoregulation function was studied in experimental animals to observe the effect of single and/or combined use with Radix Aconiti (RA) and Radix Stephaniae Tetrandrae (RST). Methods The response of delayed type hypersensitivity (DTH) in mice, the phagocytosis of abdominal macrophages (M^Φ) in mice, and the production of special antibodies in mice were observed. Results The two Chinese materia medica (CMM) in combination use could induce the increasing of phagocytosic function of M^Φ hypoimmunitic model mice, the inhibiting on the responses of DTH in the hyperimmunitic model mice and the non immunosuppressive treated mice. On the production of special antibodies in mice, the enhance effect was found in RA used in single, and the suppressive effect was found when using the combination of the two CMM. Conclusion In accordance with the mechanism of the disorder between the anti inflammation effect and the induce inflammation effect to arthritis in the immune system, these data indicate that the bidirectional effect of combination of the two CMM used on the immune responses, may be the involved mechanism on treating arthritis.

国家自然科学基金资助项目(39670868)