[关键词]
[摘要]
目的观察蜂毒素对小鼠肝癌H22细胞增殖的影响,探讨其抗肿瘤的可能作用环节。方法以MTT法测定蜂毒素体外对小鼠肝癌H22细胞增殖的影响,运用流式细胞术分析增殖细胞核抗原(PCNA)的表达,并观察iv蜂毒素对荷瘤小鼠的抑瘤作用。结果蜂毒素对H22细胞的抑制作用随药物浓度增加而增强;蜂毒素作用于H22细胞后,细胞PCNA平均荧光强度低于对照组;iv蜂毒素对小鼠皮下H22肝癌细胞具有明显的抑制作用,抑瘤作用随浓度增加而增强。结论蜂毒素在体内外对小鼠肝癌H22细胞增殖均有明显的抑制作用,下调细胞PCNA表达可能是其作用环节之一。
[Key word]
[Abstract]
Object To observe the effect of melittin on the growth and proliferation of H22 hepatocarcinoma cells and study the possible mechanisms. Methods The proliferation inhibitory rate of H22 cells treated with melittin was assayed by MTT. The volume of proliferating cell nuclear antigen (PCNA) expression of H22 cells treated with melittin was analyzed with flow cytometer after being stained with immunocytochemical methods. The antitumor effect of melittin was observed in H22 tumor burdened mice. Results The H22 cell killing by melittin was dose dependent. After treatment with melittin, the fluorescence density of PCNA of v cells decreased significantly. Melittin can inhibit the growth of H22 hepatocarcinoma in vivo. Conclusion Melittin can inhibit the proliferation of H22 cells both in vitro and in vivo Down regulating the expression of PCNA of cells may be one of antitumor mechanisms.
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[基金项目]
上海市“百人计划”项目(97BR044)