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[摘要]
目的研究不同植物生长调节剂组合对多花黄精芽体外发生过程中每块外植体上的平均芽数、叶片发生频率、根的自然发生率及生根外植体上的平均根数、根茎发生率等性状的影响;探索通过组织培养快速繁殖该植物的最佳途径。方法多花黄精的不定芽切块在MS+BA1.0mg/L+2,4-D0.5mg/L的培养基上可被诱导产生颗粒状愈伤组织,将该愈伤组织继代于不同植物生长调节剂组合的MS培养基上,2个月后观察并统计不同植物生长调节剂组合对各性状的影响。结果TDZ1.5mg/L+2,4-D1.0mg/L对芽增值最有利,平均每块外植体上可长出8.6个芽;BA1.0~2.0mg/L+NAA1.0~2.0mg/L促进叶片形成,95%以上的外植体长出形态正常的叶片;在BA2.0mg/L+NAA1.0mg/L的培养基上,根的自然发生率最高,达到51.9%,但不及单独生长素如NAA、IAA、IBA或2,4-D等以0.5~1.0mg/L对根的诱导作用,后者可使90%以上的再生芽生根,且根生长繁茂;BA2.0mg/L+2,4-D1.0~2.0mg/L组合最适合根茎生长,直径大于0.5cm的根状茎超过100%。结论多花黄精的体外快速繁殖可通过如下途径实现:TDZ1.5mg/L+2,4-D1.0mg/L诱导不定芽扩增,BA1.0~2.0mg/L+NAA1.0~2.0mg/L诱导健康叶片的发育,1/2MS+NAA,IAA,IBA或2,4-D0.5~1.0mg/L诱导再生芽生根;BA2.0mg/L+2,4-D1.0~2.0mg/L用于以繁殖药用器官为目的的根茎的生长。
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[Abstract]
Object To study the effects of plant growth regulators(PGR) with various combinations on several characteristics including numbers of per explant,production frequencies of leaves,abiogenesis of root and rhizome during in vitro bud regeneration process of Polygonatum cyrtonema Hua so as to search for the optimum in vitro regeneration process for P.cyrtonema.Methods Granular calli were induced on MS+BA 1.0 mg/L+2,4-D 0.5 mg/L by bud explant cuttings of P.cyrtonema.The calli were inoculated into MS media with various combined PGR.The characteristics described above were observed and the effects of various combined PGR wene obtained by statistics in two months.Results Among various cambined PGR,TDZ 1.5 mg/L+2,4-D 1.0 mg/L showed the best effect for the proliferation of buds BA 1.0-2.0 mg/L+ NAA 1.0-2.0 mg/L for improving of leave development,BA 2.0 mg/L+ NAA 1.0 mg/L for root production and BA 2.0 mg/L+ 2,4-D 1.0-2.0 mg/L for rhizome growth.The number of buds per explant was up to 8.6 in average,and the production rates of leaves,root,and rhizome over 0.5 cm in diameter were up to 95%,51.9% and exceeding 100%,respectively.However,the medium containing only auxin such as NAA,IAA,IBA or 2,4-D with 0.5-1.0 mg/L is much better for root induction of new buds than the original media with BA 2.0 mg/L+ NAA 1.0 mg/L on which the explants can naturally root.Conclusion The in vitro regeneration process of P.cyrtonema can be achieved by the following steps:Firstly,proliferation of buds can be realized on medium with TDZ 1.5 mg/L+ 2,4-D 1.0 mg/L.Then,normal leaves can be developed after the new buds have been transferred to medium with BA 1.0-2.0 mg/L+ NAA 1.0-2 0 mg/L.Finally,vigorous roots can be formed from regenerated buds on 1/2 MS medium with+NAA,IAA,IBA or 2,4-D 0.5-1.0 mg/L.Medium with BA 2.0 mg/L+2,4-D 1.0-2.0 mg/L can be specially spplied to improving the growth of rhizomes.the medicinal part of P.cyrtonema.
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