目的获得转基因菘蓝的分子生物学证据。方法对经卡那霉素筛选的抗性再生菘蓝苗进行聚合酶链式反应(PCR),Southern杂交和RT-PCR检测。结果PCR和Southern杂交检测结果表明,外源半夏凝集素基因已经整合到转基因菘蓝的基因组中,RT-PCR结果表明其DNA可以正常转录成mRNA。结论可以利用农杆菌介导的基因转化技术培育抗虫的菘蓝新品系。

Object To get molecular proof of transgenic Isatis indigotica Fort.Methods The regencrated I.indigotica plantlets on kanamycin-containing media were analysed with polymerise chain reaction(PCR),Southern Blot and RT-PCR methods.Results PCR and Southern Blot analysis showed that the foreign PTA gene were inserted into the genome of the transformed I.indigotica plants.Further RT-PCR showed that the foreign PTA gene could be transcripted into mRNA normally.Conclusion Pest resistant I.indigotica can be obtained through Agrobacterium-mediated transformation.