目的 研究昆明山海棠 Tripterygium hypoglaucum(Celastraceae) (THH)根部水提液对早幼粒白血病HL- 6 0细胞凋亡的诱导作用及机制。方法 通过特征性的形态学观察 ,Annexin- V标记 ,以及流式细胞仪检测中次G1 峰的形成确定 THH的诱导细胞凋亡作用 应用 Western Blotting研究 THH对 c- Myc蛋白表达的影响。结果THH根部水提液可诱导早幼粒白血病 HL - 6 0细胞凋亡。在浓度高于 18μg/ m L时 ,THH对 HL - 6 0细胞的诱导凋亡作用呈现浓度与时间的相关性。在 THH处理 2～ 4 h后 ,癌基因蛋白 c- Myc表达降低 99%以上。结论 THH抑制了 c- Myc蛋白的翻译或后翻译过程 ,从而降低了 c- Myc在细胞周期运转中的作用 ,导致大量细胞凋亡

Object To investigate the effects of the root aqueous extract of Tripterygium hypoglaucum(Levl.) Hutch (Celastraceae) (THH) on human promyelocytic leukemia HL-60 cells and its mechanism.Methods Cell growth and toxicity assays, flow cytometry, confocal fluorescence microscopy and Annexin-V labeling, and Western Blotting Assay were used. Results THH causedmorphological changes hypodiploid sub-G1 cell accumulation and induced dose- and time-dependent apoptosis in HL-60 cells at concentrations above 18 μg/mL. Western Blotting analysis showed a more than 99% decrease of the nuclear oncoprotein c-Myc after 2-4 h of THH treatment. Conclusion THH decreases c-Myc protein expression,leading to the deactivation of cell cycle progression pathways and an accumulation of hypodiploid sub-G1cells thateventually enter apoptosis.