目的 建立臭冷杉挥发油中有效成分柠檬烯的毛细管气相色谱测定方法。方法 色谱条件为 SE- 5 4毛细管色谱柱 ,程序升温 ,分流比为 4 0∶ 1,FID检测器 ,内标为萘。结果 柠檬烯浓度在 0 .6 0 132～ 1.4 0 30 8mg/ m L 呈线性关系 (r=0 .9996 ) ,平均加样回收率为 99.6 2 % (RSD=2 .4 1% )。结论 该法简便 ,灵敏 ,易操作 ,可用于臭冷杉生药的质量评价

Object To develop are liable method for determination of limonene involatile oil of Abies nephrolep is ( Trautv. ) Maxim. leaves by capillary gas chroma tograph y (CGC). Methods SE- 54 quartz capillary column was used and the temperature of column was programmed. The splitting ratio was 40∶1.The FID was use dasdetector,and the naph thalene as internal standard. Results The standard curve was linearin the concentraion range of 0.60132 — 1.40308 mg/mL ( r=0.999 6). The average recovery was 99.62% with RSD=2.41% . Conclusion The method can be used for qual it yass essment of A.nephrolep is leaves and it is rapid, sensitive, p recise and has good reproducibility.