目的 用 RAPD方法对正品和伪品大黄进行指纹图谱的研究,为正品和伪品大黄的基原鉴定提供分子依据。方法 对 RAPD反应的实验体系进行了优化,并采用个体筛选的方法筛选 12 1个引物,采用 BSA的方法筛选了 6 4个引物。结果 分别得到了 2个具有正品大黄的特征性条带的引物。结论 该方法可靠、准确、快速、重现性好,达到了在分子水平上鉴定正品和伪品大黄基原的目的。此外,该实验亦表明 BSA的方法筛选种的特征性条带的引物的效率比个体筛选引物的效率高。

Object In order to identify the resources of official and unofficial plants in Rheum L.at molecular level, a random amplified polymorphic DNA (RAPD) analysis was performed. Methods The amplification program of polymerase chain reaction (PCR) was optimized. Arbitrary decamer primers of 121 based on the individual selecting method, and 64 based on the BSA method were screened. Results Four primers among all screened primers were found to be useful to amplify specific strips to the official plants of Rheum L. Conclusion This method is reliable, accurate, quick and reproducible for authenticating the official and unofficial plants of Rheum L. at molecular level. Moreover, the experiment Results indicate that it is more effective to screen the primers using BSA method than using individual selecting method.

国家重点基础研究发展计划项目(G2000046804)