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[摘要]
目的 应用RP-HPLC(DAD)对不同产地掌叶大黄药材进行指纹图谱比较。方法 用Inertsil ODS-3分析柱,1% HAc-H2O与1% HAc-CH3CN梯度洗脱,流速1mL/min,柱温30℃,检测波长280nm,参比波长380nm。结果 建立了HPLC指纹图谱共有模式,并对其他产地药材进行了相似度比较。结论 对大黄药材中各成分均得到很好分离,可作为大黄药材的具有专属性的指纹图谱。
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[Abstract]
Object To apply RP-HPLC ( DAD) to compare the fingerprints of Rheum palmatum Linn. collected from different producing areas. Methods Inertsil ODS-3 column was used, with mixtures of 1% acetic acid-water and 1% acetic acid-acetonitrile as mobile phase in a gradient mode. The flow rate was 1 mL/min. T he temperature of column was 30℃. The wavelength of detection was 280 nm. T he reference wavelength was 380 nm. Results After the mutual mode of HPLC fingerprint was set up, comparison of similar degree to the crude drugs of other producing areas has been carried on. Conclusion According to the selected chromatographic conditions, a good fingerprint of R. palmatum has been described. The method is simple, accurate with good reproducibility. It may be practical value for the quality control of sample for R. palmatum.
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