目的 对经同工酶初步确认的4个胡萝卜与狭叶柴胡体细胞杂种进一步从分子水平上证实他们的杂种性质。方法 随机扩增多态性DNA(RAPD)分析和5S rDNA间隔序列差异。结果 4个克隆均含有双亲特征谱带及新带。结论 从分子水平上证实了他们的杂种性质。为进一步分析杂种中柴胡药效成分的含量及筛选出高药效成分含量的细胞系奠定基础。

Object Four regenerated somatic cell hybrids between carrot and Bupleurum scorzoner if olium Willd. proved by chromosome analysis were analyzed at the molecular level. Methods RAPD analysis and 5S rDNA spacer sequence were adopted. Results In the electrophoresis pattern of the PCR products the four somatic cell hybrids had the characteristic bands of two parents and new bands. Conclusion T heir hybrid nature was clarified at molecular level. This provides a firm foundation to further analyze the main active components, saikosaponin of somatic cell hybrids, and screen out efficacious hybrid cell lines.