[关键词]
[摘要]
目的 研究不同来源桑白皮东莨菪内酯含量和测定方法。方法 采用 HPL C法 ,以 Me OH- H2 O- HAc(40∶ 6 0∶ 0 .2 5 )为流动相 ,345 nm为检测波长。结果 东莨菪内酯平均回收率为 98.88%,RSD为 1.91%,线性范围为 0 .0 2 5 6~ 0 .12 8μg。不同来源最高含量为 0 .0 173%,最低含量为 0 .0 0 2 %。结论 HPL C测定法简便 ,稳定可靠 ,重复性好 ,灵敏度高。
[Key word]
[Abstract]
Object To study the contents and the determinating method of scopoletin in Cortex Mori from different sources. Methods Scopoletin was determined by HPLC. The mobile phase was MeO HH2O-HAc (40∶ 60∶ 0. 25) . Detection wav elength was at 354 nm. Results The average recovery was98. 88% with RSD= 1. 91% and the linear range was 0. 025 6— 0. 128μg. The highest content of scopoletin was 0. 017 3% and the lowest content was 0. 002% from different producing areas. Conclusion Themethod is easy, accurate, reliable with good reproducibility and high sensitivity.
[中图分类号]
[基金项目]
“九五”国家重点科技攻关项目(96-903-02-03)