目的 将外源半夏凝集素基因 (PTA )转入四倍体菘蓝以获得鳞翅目昆虫抗性。方法 构建了以 Ca MV 35 S为启动子 ,新霉素磷酸转移酶 (Npt- )为选择标记 ,带有半夏凝集素基因 PTA的 p CAMB A2 2 0 0植物双元表达载体 ,应用根癌农杆菌 EHA 10 5遗传转化四倍体菘蓝。结果 菘蓝外植体植株再生率达到 95 %以上 ,转化率有10 %。结论 建立了以 6 - BA和 NAA为主要激素组合的四倍体菘蓝离体分化再生系统和农杆菌介导的转化系统

Object Pinella Ternata Agglutinin (PTA) gene was conducted intotet raploid Isatis indigotica Fort. to get anti-pest species. Methods Agrobacterium tumefaciens mediated transformation in which the “ high toxic” strain EHA105 and the plant binary expression vector pCAMB I A2200 were adoptedunder CaMV 35S promotor and neomycin phosphorus transferred enzyme(NptⅡ) served as the selectionmarker. Results The plant regeneration rate of transgenic explants was up to 95% and transgenic frequency was 10% . Conclusion An in vitro highly effcient plantlet regeneration system and transgenic system mediated by A. tumefaciens in the main combination of 6-BA and NAA was established via cotyledonand hypocotyl segments intetraploid I. indigotica.