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[摘要]
目的 研究蓼科植物拳参的水溶性成分,并测试其DN A裂解活性。方法 利用反相层析的方法进行分离纯化,根据化合物的化学性质与光谱数据鉴定其结构。结果 自拳参根茎的60%丙酮提取物中分得10 个化合物,确定其结构分别为没食子酸(Ⅰ ) ,色氨酸(Ⅱ ) , 2, 6-二羟基苯甲酸(Ⅲ ) , (+ ) -儿茶素(Ⅳ ) ,绿原酸(Ⅴ ) , (- ) -表儿茶素-5-O-β-D-吡喃葡萄糖苷(Ⅵ ) , (+ ) -儿茶素-7-O-β-D -吡喃葡萄糖苷(Ⅶ ) , 1-(3-O -β -D-吡喃葡萄糖基-4, 5-二羟基-苯基) -乙酮(Ⅷ ) , (+ ) -儿茶素-5-O-β -D-吡喃葡萄糖苷(Ⅸ )和(- ) -表儿茶素(Ⅹ )。结论 化合物Ⅱ ,Ⅲ ,Ⅴ ~ Ⅹ为首次从该植物中分得,化合物Ⅰ ,Ⅳ , Ⅵ ,Ⅶ , Ⅸ ,Ⅹ 具有很强的DNA裂解活性。
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[Abstract]
Object To study the chemical st ructures and DNA cleavage activity of the water-soluble constituents from Polygonum bistorta L. Methods To isolate the constituents by reversephase chromatography , and characterize their structures by the analysis of chemical property and spectral data. Results Ten compounds were isolated from the 60% acetone extract of the rhizoma from P. bistorta. Their structures were elucidated as gallic acid (Ⅰ) , tryptophan(Ⅱ),2,6-dihydroy-bezoic acid (Ⅲ) , (+)-catechin(Ⅳ) , chlo rogenic acid (Ⅴ) , (-)-epicatechin-5-O-β-D-glucopy ranoside (Ⅵ) , (+)-catechin-7-O-β-D-glucopy ranoside (Ⅶ) , 1-(3-O-β-D-glucopy ranosyl-4, 5-dihydrox y-phenyl) -ethanone (Ⅷ) , (+)-catechin-5-O-β -D-glucopy ranoside (Ⅸ) and (-)-epicatechin (Ⅹ) , respectively. Conclusion CompoundsⅡ ,Ⅲ , Ⅴ-Ⅹ were isolated from the plant for the first time. CompoundsⅠ, Ⅳ, Ⅵ, Ⅶ, Ⅸ, Ⅹshowed significa nt DNA cleavage activity.
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