[关键词]
[摘要]
目的 通过 r RNA基因内转录间隔区的碱基序列测定 ,对当归进行分子水平鉴定。方法 常规提取当归种籽 DNA,利用合成的特异性引物对其 r RNA基因内转录间隔区进行套式 PCR扩增 ,将扩增产物进行碱基序列测定。结果 琼脂糖凝胶电泳证实当归种籽中 r RNA基因内转录间隔区 PCR扩增产物存在 ;经测序后得到了当归种籽 r RNA基因内转录间隔区的碱基序列。结论 r RNA基因内转录间隔区的碱基序列测定可做为分子水平鉴定植物性中药材的又一有效途径。
[Key word]
[Abstract]
Object To set up an identified standard on the level of molecule through measuring the internal transcribed spacer regions of the rRNA gene in Radix Angelicae Sinensis Methods To extract DNA from the seeds of Radix Angelicae Sinensis by conventional method, and use composed peculiar primer amplify with the internal transcribed spacer regions of the rRNA gene, to measure the base sequence of the amplified products Results It is proved by agar sugar gel electrophoresis that the PCR amplified products of the internal transcribed spacer regions of the rRNA gene exist The base sequence of the seeds of Radix Angelicae Sinensis internal transcribed spacer regions of the rRNA gene was measured Conclusion To measure the base sequence of internal transcribed spacer regions of the rRNA gene is another effective method to identify the vegetal traditional Chinese medicine on the molecular level
[中图分类号]
[基金项目]
甘肃省自然科学基金资助项目(ZS011-A25-058-Y)