目的 研究聪圣胶囊对小鼠脑缺血再灌后脑组织自由基变化的影响 ,分析聪圣胶囊抗脑缺血损伤的作用机制。方法 采用早老龄小鼠双侧颈总动脉反复缺血再灌合并尾部放血降压模型 ,应用分光光度法观察小鼠脑组织自由基的变化及聪圣胶囊对其影响。结果 聪圣胶囊可拮抗脑缺血再灌注引起的 SOD活力的下降及 MDA含量的升高 ,明显降低脑缺血再灌注引起的 NO水平及 NOS活性的升高。结论 聪圣胶囊可通过减轻脑缺血损伤后自由基产生 ,降低升高的 NOS活性来发挥抗脑缺血作用。

Object To investigate the effects of Congsheng Capsule (CSC) on free radical change after cerebral ischemia reperfusion, and analyze the mechanisms of CSC anti cerebral ischemia action. Methods Bilateral carotid artery occlusion and reperfusion combined with tail bleeding hypotension were employed in this study. Changes in free radicals were observed by means of spectro photometry. Results CSC 1, 3, 9 g/kg could enhance SOD activity, reduce the MDA content, and decrease NOS activity and NO content. Conclusion The protective effects of CSC on cerebral ischemia may be mediated by its anti free radical and NO damage.

“九五”国家攻关课题(96-906-09-03)