目的 研究苦参碱诱导 K5 6 2细胞分化的信号转导机制。方法 运用链亲和素-生物素放大系统结合的酶联免疫法 ,动态检测苦参碱作用于 K5 6 2细胞后 ,K5 6 2细胞膜相与胞浆内的酪氨酸激酶与磷酸酶的变化。结果 结合特异性激酶抑制剂的使用 ,首次证实了在苦参碱对 K 5 6 2细胞的诱导分化过程中 ,有广泛性的蛋白酪氨酸激酶活性的短暂下降 ,同时伴有蛋白酪氨酸磷酸酶的活性变化。结论 在苦参碱诱导 K5 6 2细胞分化的信号转导过程中 ,涉及到蛋白酪氨酸激酶的活性改变 ,膜相中蛋白酪氨酸激酶的活性改变先于胞浆内的改变 ,提示有一个信号的跨膜转运过程。同时伴有蛋白酪氨酸磷酸酶的活性变化 ,反映了胞内的蛋白酪氨酸残基磷酸化与去磷酸化的即时调节机制。

Object To study the mechanism of signal transduction in K562 cell differentiation induced by matrine. Methods The method of ELISA coupled with streptaviding biotin system was used to detect the activity of protein tyrosine kinase and phosphatase respectively in cytoplasm and membrane of K562 cells treated by matrine at different time. Results Using specific kinase inhibitor, it was demonstrated in the first time that the activity of protein tyrosine kinase decreased transiently accompanying the change of protein tyrosine phosphatase activity. Conclusion The change of protein tyrosine kinase activity was involved in the course of K562 cell differentiation induced by matrine. The tyrosine kinase activity in cell membrane decreases more rapidly than that in cell cytoplasm, suggesting that there be a signal transmembrane transport process. The change of tyrosine phosphatase activity following the kinase reflects the real time regulation of phosphorylation and dephosphorylation.

国家自然科学基金资助项目(39670881;30171150)