目的 了解蜂毒素对肝癌细胞生长、增殖的影响。方法 采用台盼蓝排染法测定细胞的生长曲线 ,MTT法测定细胞增殖的抑制率 ,流式细胞仪检测细胞周期和细胞 PCNA 的表达。结果 蜂毒素可明显抑制肝癌细胞SMMC772 1的生长、增殖 ,PCNA阳性细胞表达率随着蜂毒素剂量的增加而降低 ,对细胞周期的影响表现为 S期细胞增加 ,G2 / M期细胞减少。结论 蜂毒素可抑制肝癌细胞的生长、增殖 ,并且减少 PCNA阳性细胞的表达 ,影响细胞周期的比率。

Object To study the effect of melittin on growth and proliferation of hepatocarcinoma cells. Methods Hepatocarcinoma cell line SMMC7721 was treated by melittin. The growth and proliferation was observed by cell counting and MTT assay; necrosis was estimated by Trypan blue staining. The expression of proliferating cell nuclear antigen (PCNA) and cycle phase were detected by flow cytometer. Results Melittin could significantly inhibit the growth of SMMC7721 and block up cells progress through G 2/M. The percentage of PCNA positive cell was decreased gradually when concentration of melittin was increased. Conclusion Melittin could inhibit the proliferative activity of hepatocarcinoma cell line SMMC7721 and reduce of PCNA positive cell expression and arrest cells in S phase.

军队“九五”重点课题(98DJ-3269)