目的 为了有效加速贵重药用植物金钗石斛的人工繁殖。方法 以带侧芽的金钗石斛嫩茎段作外殖体;不同种类的培养基、同种培养基用不同配比的激素进行培养。结果 以 MS培养基附加 6 - BA (0 .5 mg/ L )、NAA(0 .2 m g/ L)诱导分化最佳 ,附加 6 - BA (3.0 mg/ L)、NAA (0 .5 mg/ L)增殖最适 ,附加 IBA(0 .3mg/ L)、NAA(0 .1mg/ L )对生根最适 (生根率 95 % )。生根小苗在以椰渣、碎火山石块、碎砖块 (1∶ 1∶ 1)的基质中覆以干蕨长势良好。结论 为确保金钗石斛资源的保持和可持续利用提供有效途径。

Object To speed up the artificial propagation of the costly medicinal plant Dendrobium nobile Lindl. Methods The tender stems with lateral buds were tried as the explants and cultured on various culture media with different portions of hormon. Results The best medium for bud induction was the MS basic medium with addition of 6 BA (0.5 mg/L), NAA (0.2 mg/L), while MS with the addition of 6 BA (3.0 mg/L) NAA (0.5 mg/L), was suitable for propagation, MS with addition of IBA (0.3 mg/L), NAA (0.1 mg/L) was better for root growing, the striking growth rate was up to 95%. The young sprout was cultured fine on the base material mixed with coco crumbs, volcanic rocks and brick fragments (1∶1∶ 1) and covered by dried pteridophyte. Conclusion To provide the effective ways to reserve the resources of D. nobile and last its utlization.