目的 确定杭白菊总黄酮的最佳提取工艺 ,并考察总黄酮含量随杭白菊不同采摘时间的动态变化。方法 采用正交试验法 ,考察提取温度、提取时间、乙醇浓度及提取次数对提取液中总黄酮含量的影响 ,确定最佳提取条件 ,然后在该条件下对同一种植区域 ,不同时间采摘的杭白菊进行总黄酮提取并进行含量测定。结果 在所考察的因素中 ,对杭白菊黄酮提取影响程度为 :乙醇浓度 >提取温度、提取次数 >提取时间 ,不同时间采摘的杭白菊 ,其总黄酮含量未见明显变化。结论 杭白菊黄酮最佳提取条件为 :75 %乙醇 ,10 0℃水浴回流提取 3次 ,每次 1.5 h;杭白菊总黄酮含量与采摘时间无关。

Object To optimize the extraction conditions for flavonoids from Chrysanthemum morifolium Ramat., and observe the variety trends of flavonoids during collection time. Methods Optimized conditions (bath temperature, extraction hours, ethanol concentration and extraction times) were determined by the orthogonal test. The flavonoids in C. morifolium collected at different time were measured under the optimized extraction conditions. Results The order of factors to affect the flavonoid extraction was ethanol concentration bath temperature, extraction times extraction hours. No significant difference of flavonoids content was observed among the different collecting time of C. morifolium. Conclusion The optimum extraction conditions were 75% ethanol, refluence for 1 5 h at 100 ℃ water bath for three times. The flavonoids would not be bound up with the harvest time.

浙江省教育厅资助项目(G20062);浙江省桐乡市科委资助项目(H20000792)