[关键词]
[摘要]
目的 探讨百部的组培快繁技术,为优良品种的快速繁殖奠定基础。方法 以带侧芽百部茎段为外植体 ,采用MS为基本培养基 ,附加不同植物生产调节剂进行试验。结果 采用 MS+6-BA3.0mg/ L+IBA 0.2mg/ L的培养基能成功地诱导芽的分化 ;对于芽增殖 ,以MS+6-BA3.0mg/ L+IBA0.3mg/ L 培养基较好 ;诱导生根以MS+IBA2.0 mg/ L +AgNO30.5 mg/ L培养基较好 ,培养30d后生根率可达 50%以上;20mg/ L多菌灵处理可明显提高移栽成活率。结论 初步建立了百部的组培快繁体系 ,使工厂化生产百部种苗成为可能。
[Key word]
[Abstract]
Object To apply the technique of tissue culture to the rapid propagation of Stemona japonica(Bl.) Miq.and lay afoundation for the rapid propagation of its improved breeds. Methods The explants used for culture were stem segments with axillary bud of S.japonica. The media were MS basal media containing different plant hormones. Results The results of the study indicated that shoots could be induced successfully in MS medium supplement with 6-BA 3.0 mg/L and IBA 0.2 mg/L, and the better medium for bud multiplication and root inducement were MS+6-BA 3.0 mg /L+ IBA 0.3 mg /L and MS+IBA 2. 0 mg /L+ AgNO3 0. 5 mg /L respectively. After 30 days 'culture, the rate of rooting reached over 50% . The survival rate of transplanting was raised obviously by using 20mg /L carbendazim treatment.Conclusion The primary plant regeneration system of S.japonica has been established, which makes the industrial production possible.
[中图分类号]
[基金项目]
国家林业局中南速生材繁育重点实验室资助;广西大学科研基金资助(X98225)