[关键词]
[摘要]
目的 研究室内菲牛蛭生长动态和生殖,为人工饲养菲牛蛭提供基础资料。方法 在同一个卵袋产下的幼蛭 ,放入盛有天然水的80cm×60cm×50cm的玻璃缸内饲养 ,每10d称牛蛭体重1次 ,每天记录水温 ,每月换水一次 ,每次在野外采回的水要检测10种化学成分。在活兔身上吸血 ,每次称出吸血量。观察生长发育变化、记录交配产卵过程。结果 从孵化出的幼蛭经14个月时间 ,吸血5 ,6次 ,生长发育达到性成熟,第1,2 ,3次吸血为幼体阶段,第4次吸血后进入亚成体阶段 ,第5次吸血后 ,部分个体达到性成熟 ,有的个体第6次吸血后进入性成熟。生长动态曲线是一跳跃曲线。记录了交配、产卵过程。结论 阐明牛蛭生长动态和生殖过程。在室内饲养牛蛭成功。
[Key word]
[Abstract]
Object To study the dynamic growth and reproduction of Hirudinaria manillensis Lesson in laboratory with providing the data of artificially breeding leech in the field. Methods The newly hatched juvenile from same cocoon were bred in 80cm×60cm×50 cm aquaria with natural water from rice paddy and weighted every ten days. Water tempera ture was recorded daily and water was exchanged monthly. Ten chemical elements of water from rice paddy were analyzed in the laboratory. Leeches were allowed to engorge the blood on living rabbits and the amount of engorged blood was measured every time to observe the growth and development , record the copulation and cocoon-deposit , and make the curve of dynamic growth of the leech. Results The newly hatched juvenile grew and developed for 14 months to sexual maturation period by engorging rabbit 's blood of five or six times. The leeches were still juvenile in the first three times of engorging, became subadult in the fourth. Then some developed to the sexual maturation period individually in the fifth , and some individually in the sixth. The curve of dynamic growth displayed rising and falling with the record of copulation and cocoon deposit. Conclusion The above study elucidate the dynamic growth, reproduction and the successfully breeding of leeches in the door.
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[基金项目]
国家自然科学基金资助项目(3880068,39160017);广东省中医药管理局基金(200011)