[关键词]
[摘要]
目的 优选分离栀子苷的大孔树脂。方法 将栀子粗提液置大孔树脂中吸附,并以水、70 %乙醇分别洗脱 ,以HPLC法测定洗脱液中栀子苷含量。结果 D301R、D396、D296和AB-8四种型号大孔树脂效果差异较大。结论 D301R型大孔树脂用作栀子苷的分离效果较好。
[Key word]
[Abstract]
Object To optimize the macropo rous resin for sepa rating gardenoside. Methods Putting the extract fluid of Gardenia jasmincides Ellis into pillar was adsorbed with macroporous resin, then washed by water, 70% alcohol in succession to determine the content of gardenoside by HPLC. Results D301R, D396, D296, and AB-8, the four macro porous resins ' effects were different a lot. Conclusion D301R macroporo us resin is effective to separate the gardenoside.
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[基金项目]
国家中医药管理局中医药科学技术研究基金资助项目(国中医药科00-01KZ01号)