[关键词]
[摘要]
目的体外观察牛蒡苷元 (arctigenin,ACT)抗甲 1流感病毒作用,为进一步确认 ACT是牛蒡子 Fructusarctii解表功能有效成分提供实验依据。方法采用 MDCK细胞培养法,通过红细胞凝集实验,以流感病毒血凝滴度为指标,观察 ACT对流感病毒复制的抑制作用。结果 ACT浓度 (m mol/ L)为 6.7,13.4,2 6.8和 5 3.6的血凝滴度抑制率 (%)分别为 0.75,87.5和 10 0。结论 ACT在体外有直接抑制流感病毒复制的作用,是牛蒡子解表功能的有效成分,对其进行含量测定作为牛蒡子质量标准的定量指标是适宜和可行的。
[Key word]
[Abstract]
Object To observe in vitro inhibitory activity of arctigenin (ACT) against influenza A1 virus in order to make further verification of that ACT is an active constituent of Fructus Arctii for relieving exterior syndrome. Methods Madin darby canin kidney (MDCK) cell culture techniques were used for evaluating antiviral activity of ACT by hemagglutination assay with the influenza virus hemagglutination titer as index and for investigating the inhibitory effect of ACT on replication of influenza virus. Results The inhibition rates (%) of hemagglutination titer were 0.75, 87.5, and 100 at the ACT concentrations (mmol/L) of 6.7, 13.4, 26.8 and 53.6, respectively. Conclusion ACT has directly in vitro inhibitory effect against influenza virus' replication, and is an active constituent of Fructus Arctii for the relieving exterior syndrome. It is suitable and feasible to determine the quality control index as ACT content in Fructus Arctii to assess the quality of the herbal drug.
[中图分类号]
[基金项目]
“九五”国家重点科技攻关项目(No.96-903-02-01)