[关键词]
[摘要]
目的为获得高质量的DNA,研究木质部较发达的根、根茎类以及茎木类药材的DNA提取方法.方法总DNA的提取过程包括用Tris缓冲液洗净、CTAB抽提和纯化.结果对升麻、楤木、木香、乌药、芍药和木通等进行了DNA提取,能较好的去除色素、多糖等干扰PCR物质.结论本法使用常用试剂,成本低,易于推广,为药材DNA分子鉴别的应用奠定了基础.
[Key word]
[Abstract]
ObjectTo explore the method for high-quality DNA extract from plant Chinese medicinal materials containing developed xylem. MethodsThe process of DNA includes wash with Tris-buffer,extractwith CYAB-buffer and purification mainly. ResultsUsing this method,the total DNA was isolated from Cimicifugae foelida L., Arlia chinensis L.,Paeoniae lactiflora Pall.,Aucklandialappa Decne.,Linderaaggregata(Slms)Kostern.,and Akebia quinata Decne.etc. This method is preferable in removing pigments,polysaccharides, substance disturbing PCR. ConclusionIn this process, commonreagents are used,lower cost is of advantage to popularize. This study provids the applying foundations for the DNA identification of plant Chinese medicinal materials.
[中图分类号]
[基金项目]
中国博士后科学基金(1998年)