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[摘要]
目的研究百合科植物麦冬 Ophiopogon japonicus及其同属植物沿阶草 O.bodinieri四川所产 10个居群间的遗传多样性。方法以40个10碱基随机引物进行 RAPD分析。结果11个有效引物扩增获得 5 15条 DNA带,通过聚类分析,构建 DNA分子系统树图,确定了麦冬及沿阶草的居群及种间亲缘关系。结论(1)供试样品间遗传差异明显。具有丰富的遗传多样性,但是其遗传差异与地理分布联系并不紧密,而与形态差异联系较紧 ;(2 )麦冬与沿阶草之间存在着较大的遗传差异,亲缘关系较远
[Key word]
[Abstract]
ObjectTo assess the genetic diversity among ten natural populations in Ophiopogon japonicus(L.f.) Ker-Gaw l.and O.bodinieri Levl.(Li liaceae) in Sichuan Province. MethodsForty random decamer primers were screened for Random Amplified Polymophic DNA(RAPD) fragments. ResultsBased on cluster analysis of 515 DNA bands amplified by 11 primers, a DNA molecular dendrogram was established, and the relationship of the populations and seeds between O.japonicus and O.bodinieri was set up. Conclusion1. Distinct genetic differences and extensive genetic diversity are presented among the samples. The genetic differences are related to morphological differences, but not to geographic regions;2.Quitegenetic differences are presented between O.japonicus and O. bodinieri,it suggests that they have farther relationships.
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四川省重点项目