目的研究自拟中药复方"五子四物瓜石汤"(WT)对雷公藤多苷(GTW)所致雄性大鼠生殖系统毒性的对抗作用及其机制.方法雄性大鼠分别ig大、中、小剂量的WT与固定剂量的GTW,连续给药80 d,以单独igGTW或NS为对照,观察各组雄性大鼠睾丸脏器指数、精子数、精子活率、睾丸病理结构的变化,并用免疫放射分析法(IRMA)测定各组雄性大鼠血清性激素水平.结果与单用GTW组比较,大或中剂量WT可使睾丸重量明显增加,精子数明显升高,精子活率显著提高,其中大剂量WT可完全对抗GTW对生殖上皮的损害,使睾丸结构完全正常,血清睾酮维持于正常水平.结论"五子四物瓜石汤"能显著对抗雷公藤多苷对雄性大鼠生殖系统的毒性作用,其对抗作用与wT保护睾丸生精上皮免受GTw的损害,并使血清睾酮水平维持于正常有关.

ObjectTo study the effects of WUZI SIWU GU ASHIT ANG (WT) against multi-glyco-sides of Tripterygium wilfordii Hook.f.(GTW) -induced genital system toxicity in malerats and its mechanism. MethodsMale rats in treatment groups were treated respectively with a 80-days oral administration of large-, middle- and small-doses of WT plus a fixed dose of GTW. The rats as control weret reated with GTW alone or saline. Thereafter, the changes in testisorg an index, the number of spermatozoa, the rate of movable spermatozoa and the structure of spermatogenic cell epithlium were observed and the serum testostero nelevel was determined by IRMA. Then the comparison was made between treatment and control groups as to above variables. ResultsAs compared with GTW group,large- or middle-dose of WT significantly increased the weight of testis, the number of spermatozoa and the rate of movable spermatozoa. Large dose of WT could relieve the damage of GTW to the spermatogenic cell epithelium completely and keep the serum testosterone at normallevel. ConclusionThe combined use of GTW with WT is very useful in relief of GTW-induced adverse reactions of genital system in male rats. The mechanism by which WT exerts the counteractive effects is related to protecting the spermatogenic cell epithelium from being damaged by GTW, and keeping the serum testosterone at normal lev el.

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