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[摘要]
目的观察青心酮对红细胞及肺血管内皮细胞生物力学特性的影响。方法体外培养肺动脉内皮细胞,分对照组及青心酮大、中、小剂量处理内皮细胞,用微管吸吮技术观察细胞的变形过程。结果与未用药组比较,在加用青心酮 30 min后,中、大剂量组内皮细胞弹性系数 K1 和 K2 增大,粘性系数μ变化不大 ;红细胞表观变形指数和最大变形值也明显增加。结论青心酮可以增加肺血管内皮弹性,并使红细胞变形能力明显增强,这可能是青心酮改善血液循环的生物力学机制
[Key word]
[Abstract]
ObjectTo observe the effects of 3,4dihydrox yacetophenol(DHAP) on biomechanic properties of cultured pulmonary artery endo thelial cells a nd i sola ted ery th rocy tes.MethodsCultured in vitro pulmonary artery endoth elial cells were treated with large-, middle-and small-doses of DHAP, and a control group was set up. The deformation process was observed using micropipet teaspiration technique. ResultsCompared with the control group, the elastic coefficiency K1 and K2 of endothelial cells in large-and middle-dose groups increased significantly, while the viscosity coefficiency changed insigni ficantly in 30 min of giving DHAP. The apparent deformation index and maximum deformation value of erythrocy tesalsoincreased markedly. ConclusionDHAP can increase pulmonary blood vessel endothelial elasticity and erythrocy tedeforma bility obviously, thus improving the blood circulation.
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[基金项目]
国家自然科学基金资助项目(No.30070929)