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[摘要]
目的 探讨天南星族药用植物间生理生化特征的相关性和差异性,分析它们的亲缘关系,为其分类鉴定提供科学依据.方法 应用聚丙烯酰胺凝胶电泳技术对天南星族植物11种5变种1变型共29个样品进行蛋白质电泳分析,从电泳谱中取49个数量化特征,以单链法对这类植物进行聚类分析.结果 同种植物的蛋白质电泳谱较相似,而不同种植物之间有明显差异.结论 (1)此类植物的蛋白质电泳谱具有一定的稳定性和可靠性,(2)蛋白质电泳分析结果与传统经典分类结果基本吻合,(3)长管半夏Pinellia longitubulosa应建立新种;粉背盾叶半夏P.peltata var.chunanensis和绛斑湘南星Arisaema hunanense var.ningPoense宜成立新变种;全缘灯台莲Arisaemasikokianum、灯台莲A.sikokianum var.serratum及七叶灯台莲A.sikokianum var.henryanu 3变种应予归并.
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[Abstract]
Object To discuss the correlation and difference of physiological and biochemical charaeteristic among the plants of the tribe Areae in genotypes, to provide seientific evidences for the classification and identification of their consanguinity Methods Protein electrophoresis analysis in the fresh tuber of 29 samples from 11 species, 5 varieties, 1 new form of the tribe Areae in Zhejiang Province was done by polyacrylamide gel electrophoresis Based on the 49 numerical characters obtained from their electrophoretograms, cluster analysis of the plant was carried out in single linkage method Results The electrophoretograms of the same species were quite similar, but those of the different species were clearly distinct from each other Conclusion 1 The protein electrophoretograms of these plants exhibited certain reliability and stability. 2. The Results of protein analysis were accordance with those from the classical taxonomy on the while 3 Pinellia longitubulosa H.X.Sun should be estabilshed as a new species.P.peltata Pei var.chunarensis H.X.Sun and Arisaema hunanense Hand.-Mazz var ningpoense H.X.Sun should be considered as new varieties, respectively, their electrophoretograms were obviously different from those of original varieties Arisaema sikokianum Franch et Sav., A.sikokianum Franch et Sav.var serratum Makino H.Li should not be kept as independent varieties, which should be incorporated with A.sikokianum var. henryanu.
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