[关键词]
[摘要]
目的 鉴别中药材厚朴、凹叶厚朴及其常见的伪品和混淆品.方法 采用任意引物PCR(arbitrarilyprimed PCR,AP-PCR)技术扩增植物基因组DNA样品.结果 AP-PCR技术获得清晰可靠的DNA指纹图谱,根据琼脂糖凝胶上显示的DNA带型差异可迅捷地区分厚朴、凹叶厚朴及其伪品、混淆品.结论 为应用AP-PCR技术在分子水平上鉴别中药材厚朴,保证引种的准确性奠定了基础.
[Key word]
[Abstract]
Object To identify Magnolia officinalis Rehd et Wils and M biloba Rehd et Wils as well as their counterfeits and easily confusable substitutes Methods Total genomic DNA samples of ten plant species were amplified by arbitrarily primed PCR (AP-PCR) Results Ten samples were distinguished through their amplified DNA banding patterns on the agarose gels after eletrophoresis, so as to differentiate the counterfeits and easily confusable substitutes of M officinalis and M biloba. Conclusion AP-PCR is useful for identifying M officinalis at molecular level, as well as valuable to guarantee introducing original plant correctly
[中图分类号]
[基金项目]