[关键词]
[摘要]
目的研究开唇兰小菇、石斛小菇、兰小菇等3种小菇属内生真菌对兰科濒危药用植物铁皮石斛、金线莲生长的促进作用.方法 采用植物无菌原球茎及试管苗与真菌进行双重培养,观测原球茎增殖及苗的生长情况.结果 接种3种内生真菌后,铁皮石斛苗的生长量高于对照3～5倍,石斛小菇、兰小菇对铁皮石斛原球茎增殖也有明显促进作用(P＜0.05);接种3种真菌的金线莲苗,侧芽及侧根数均显著高于对照.结论 3种小菇属内生真菌能够促进铁皮石斛幼苗生长,促进金线莲侧芽及侧根的萌发,对铁皮石斛、金线莲的成功栽培具有实际应用价值.
[Key word]
[Abstract]
Object To study the promoting effects of Mycena anoectochila, M dendrobii and M. orchidicola on growth of Dendrobium candidum Wall. ex Ldl. and Anoectochilus roxburghii (Wall) Ldl Methods The protocorm and plantlet of D candidum and A roxburghii were grown in dual culture with endophytic fungi to observe the proliferation of protocorm and the growth of plantlet. Results The growth of plantlet of D candidium, which was inoculated three endophytic fungi, increased markedly 3 5 times more than those of the control one M dendrobii and M orchidicola also promoted multiplication on protocorm of D candidum (P0 05). After inoculated three endophytic fungi, the proliferation of plantlet, new buds and lateral roots of A roxburghii are getting more than those of the control one Conclusion Three endophytic fungi, M. anoectochila, M. dendrobii, and M. orchidicola, could promote the platelet growth of D. candidum and increase the number of buds and roots of A. roxburghii. It has great value for culture of D. candidum and A. roxburghii.
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[基金项目]
高等学校全国优秀博士学位论文作者专项基金(199950);国家自然科学基金(39970018);高等学校博士点学科点专项科研基金资助项目(1998002337)
