目的考察四逆汤有效成分不同组合抗心肌缺血再灌注损伤的作用。方法采用正交试验法,以超氧化物歧化酶(SOD)、丙二醛(MDA)以及乳酸为指标,考察附子生物碱(A)、干姜挥发油(B)、甘草酸粗品(C)3个因素。结果3种有效成分最佳剂量为A₄B₄C₄,3因素对SOD和MDA影响大小顺序为A>C>B,对乳酸影响大小顺序为A>B>C。结论3种有效成分临床极量组合的疗效最佳。附子生物碱是四逆汤有效成分组合中的关键因素,干姜挥发油和甘草酸粗品也是组方中不可或缺的因素。

Object To study the protective effect of SINI TANG comprising active components combined at different dosage on myocardiali schemia reperfusion injury in mice. Methods In the studies,the experiment was performed by orthogonal design,the levels of SOD, MDA and lactate in cardiacmuscle were regarded as criterion for evaluation of the every composition, three active components were studied including the alkaloid(A)from Aconitum carmichaeli Debx.,the volatile oil (B)from Zingiber of ficinale Rosc.and crudeglycyrrhizic acid(C)from Glycyrrhizauralensis Fisch. Results The optimalcompo sition comprised A at dose of 31.2 mg/kg,B at dose of 1.84μL/kg and C at dose of 4.68 mg/kg(A₄B₄C₄).The effect of three active components on SOD and MDA was A> C> B and lactate was A> B> C.Conclusion The optimal SINI TANG composition comprises three active components combined at clinical maximaldosage showed the best therapeutic efficacy,of which A is a key factor and B,C are necessary factors for the composition in SINI TANG.

中山大学“211工程”重点建设项目(98176);广东省中医药管理局科研基金项目(100118)