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[摘要]
目的 研究桑白皮具有专属性的薄层色谱鉴别法。方法 用薄层色谱法,取桑白皮与混淆品种根皮的乙醇提取物,选择不同的展开剂展开,在紫外灯下检视荧光或三氯化铁显色,找出桑白皮特有斑点。结果 以CHCl3-MeOH(5∶1)为展开剂,三氯化铁显色,桑白皮色谱中,在与混淆品种色谱相应位置上,显一个特有的紫色斑点。结论 特有斑点可作为桑白皮专属性的定性对照品,波谱分析鉴定为桑根酮C。
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[Abstract]
Object To study specific TLC identification of the root bark of Morusalba L.(RBMA) Methods The ethano lextracts of RBMA and other confusable species were subjected to TLC in different development system. The TLC plats were examin edundershort wave length UV lightor colored by FeCl3<\sub> solution.Results A mixture of chloro form and methanol(5∶1)is used for development and ferric chloride solution is used to colo r, a specific purple spot of the certified RBMA can be found. Conclusion The specificspot showed with TLC can beregarded as the basis of identification of RMBA and it was separated an didentified as sanggen on C.
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“九五”国家重点科技攻关计划(96-903-02-03)