目的 研究粉防己碱(Tet)对有机磷酸酯抑制的胆碱酯酶(ChE)活性的影响。方法 采用Ellman’s比色法测定人血ChE活性作为观察指标。结果 Tet使敌百虫抑制的ChE活性量效曲线右移,最大抑制反应明显压低;0.4,0.8,1.6μg/mLTet的最大复活效应分别为20.5%、38.3%和80.2%(P<0.05);Tet与氯磷定(PAM-Cl)活化被抑制酶的EC50分别为0.69和2.01μg/mL。结论 提示Tet是被抑制的ChE复活剂。

Object To observe the effect of tetrandrine(Tet)on the activity of cholines terase(ChE) in hibited by organophos phate.Methods Activity of ChE in human blood was deter mined with Ellman's method and used as anindex.Results The cumulatived ose-effect curves for inhibit ory effect of dipterex on ChE were shifted to right and the maximal inhibitory effect of dipterex was obviouslyde clined by Tet. The maximalre activating rates of inhibited ChE were 20.5%,38.3% and 80.2% when the concent rations of Tet were 0.4,0.8 and 1.6μg/mL(P < 0.05),respectively.The EC 50 values of Tet and PAM-Cl for reactivation of ChE were 0.69 and 2.01μg/mL,respectively.Conclusion It was suggested that Tet is are activator of ChE inhibited by organophos phate.

国家自然科学基金资助项目(No.39760082)