目的 测定显齿蛇葡萄中杨梅素的含量。方法 采用 RP- HPLC法测定,色谱柱为 Nova- Pak C₁₈不锈钢柱,检测波长为 2 5 4nm,用甲醇 -水 =40:60为流动相。结果 方法的变异系数为 2.763%,平均回收率为 97.4%,最低检测量为 15 ng。湖南显齿蛇葡萄植物中不同部位的杨梅素含量为 1.5 7%～ 2 .17%。结论 方法简单、快速、精确,可作为显齿蛇葡萄植物质量检测方法之一.

Object To establish a method for the assay of myricetin in Ampelopsis grossedentata (Hand. Mazz.) W. T. Wang. Methods The determination was carried out with RP HPLC on Nova pak C 18 stainless steel column (150 mm × 3 9 mm) with methanol∶water (40∶60) as the mobile phase, and detected at a UV wave length of 254 nm. Results The coefficient of variation was 2.763% with average recoveries=97.4%. The minimal detectable limit was 15 ng. Contents of myricetin in different parts of A. grossedentata from Hunan Province varied from 1.57% to 2.17%. Conclusion The method is rapid, simple, accurate and good for the determination of myricetin in A. grossedentata.

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