目的 从 DNA分子水平上探讨当归药材道地性的生物学基础。方法 对来自不同产地的 18个当归样品 ,提取其基因组 DNA,并进行 RAPD分析。对筛选出的 10个引物的 RAPD结果进行聚类分析 ,得出 18个当归样品的聚合树状图。结果 显示地理分布距离越小 ,当归的遗传差异越小 ;反之 ,越大。但生态环境特别是小生境对当归遗传差异的作用亦不可忽视。结论 为我们从分子生物学角度研究当归药材道地性提供了新的启示

Object To investigate the genuineness of Angelica sinensis (Oliv.) Diels from the level of molecular biology. Methods The genomic DNAs of 18 samples of A. sinensis collected from different localities were isolated and analysed by RAPD. Based on the cluster analysis of their RAPD strips, the molecular phylogenetic tree of 18 A. sinensis samples was described. Results The rusults indicated that the smaller the geographic distance between two A. sinensis samples, the smaller their genetic difference; while the genetic difference became greater as their geographic distance increases. However it should not be overlooked that the natural environments, especially the local ecological conditions may also show some influence on the genetic variations among different A. sinensis samples. Conclusion It has provided us with the enlightenment of study on the genuineness of A. sinensis from the level of molecular biology.

国家自然科学基金重点项目(编号39730500)