目的 从整体水平探讨瑞香狼毒水提物(SCLA)的抗癌作用及机制。方法 以不同剂量SCLA ig给药后，不同时间采集小鼠血清，处理体外培养的小鼠白血病L₁₂₁₀细胞，观察肿瘤细胞MTT还原，克隆形成能力和DNA合成的改变。结果 SCLA 3，6，12g/kg给药1，2，4，8h后的药物血清处理肿瘤细胞后，其MTT还原及克隆形成率均显著降低；给药2h时的药物血清表现出更强的抑制肿瘤细胞增殖作用。SCLA小鼠药物血清也显著抑制[³H]TdR掺入L₁₂₁₀细胞DNA。结论 直接抑制癌细胞增殖及DNA合成是瑞香狼毒的重要抗癌机制。

Object To explore, on the whole, the anticancer activity and mechanism of the aqueous extract of Ruixiang Langdu (Stellera chamaejasme L.) (SCLA) . Methods Mice were given different i. g. doses of SCLA and their serum collected at different intervals after drug administration. The effects of the serum on the proliferation of mouse L₁₂₁₀ leukemic cells were observed with MTT assay, clone formation and incorporation of [³H] TdR into DNA of the cells. Results Serum SCLA collected 1, 2, 4, 8 h after administration of 3, 6, and 12g/kg SCLA showed significant decrease of MTT formazans and clone formation. Serum SCLA collected 2 h after drug administration showed more strong inhibition of cancer cell proliferation. It also inhibited the incorporation of [³H] TdR into DNA of L₁₂₁₀ cells. Conclusion SCLA showed a remarkable inhibitory effect on proliferation and DNA synthesis of cancer cells cultured in vitro,which may be the main anticancer mechanisms of SCLA.