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[摘要]
目的 从双齿围沙蚕体内提取并纯化一种新的纤溶酶。 方法 应用硫酸铵沉淀、透析、凝胶柱层析(Sephadex G-100)以及电泳等生化手段对沙蚕纤溶酶进行分离纯化。 用平板法测定它降解纤维蛋白的活性。 结果 得到纯的沙蚕纤溶酶, 测出其等电点为4.5左右, 由两条肽链组成, 其相对分子量分别为33000u和14400u。 结论 该酶为首次从沙蚕中发现的一种新的纤溶酶。
[Key word]
[Abstract]
Object To extract and purify a novel fibrinolytic enzyme from Perinereis aibuhitensis Grube. Methods The enzyme was precipitated f rom the extract by ammonium sulfate, dialyzed, chromatographed on Sephadex G-100 column, and then purified by polyacrylamidegel electrohoresis. Its fibrinolytic activity was assessed with fibrin plate method. Results The purified enzyme showed an isoelectric point(PI) around 4.5 as tested by gelisoelectric focusing. It consisted of two polypeptide chains with molecular weight saround 33000u and 14400u, respectively. Conclusion This was a novel fibrinolytic enzyme discovered from P. aibuhitensis for the first time.
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[基金项目]
国家海洋863资助项目(819-Q-16)