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[摘要]
目的 研究百合组培快繁的最佳途径。方法 在 MS培养基上添加不同的激素配比 ;采用鳞叶不同部位作为外植体 ;改变培养方式。结果 确定了外植体产生小鳞茎的最佳培养基为 MS+ NAA 0.5 mg/L + GA32.0mg/L;鳞叶的下部小块分化率最高 ,为 92.5 % ;小鳞茎叶脱分化形成愈伤组织后再分化可获得多而健壮的次级小鳞茎 ;次级小鳞茎采用液体振荡培养可快速增重。结论 采用组织培养方式可进行百合的快速繁殖 ,使百合种球的工厂化生产成为可能。
[Key word]
[Abstract]
ObjectTo develop a best approach for the rapid propagation of Lilium brownii F. E. Brown var. viridulum Baker by tissue culture. Methods Different parts of the bulb w ere tried as the explant and cultured on different culture media with the additon of dif ferent portins of vario us hormones at vario us cultural conditions. Results The best medium for the culture of explant bulb was MS+ N AA 0. 5 mg/L and GA3 2.0 mg/Land the hig hest induction rate was at the lowest part of the scale leaves, which attained 92. 5% . The small bud can further differentiate to form secondary small buds. By liquid-quiv ering culturing , the weight increase can be accelera ted. Conclusion Tissue culture of L. brownii var. viridulum can achieve its rapid propagation, resulting in the possibility for its industrial production.
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[基金项目]
江西省一级重点研究项目