[关键词]
[摘要]
目的 运用 HPLC建立金森脑泰粉针剂中三七皂苷 R1 的含量测定方法。方法 采用 ODS色谱柱 (5 μm,2 5 0 mm× 4.6 m m),流动相为甲醇 -水 (5 5∶ 45),流速 1m L /min,检测波长 :203 nm。结果 该法回收率 97.0 0 %,RSD=1.6 0 % (n=5)。结论 本法操作简便、准确,为测定金森脑泰粉针剂中三七皂苷 R1 含量提供了可靠方法。
[Key word]
[Abstract]
Object To establish a method for the determination of notoginsenoside R 1 in JINSENNAOTAI POWDER INJECTION by HPLC Methods The determination was carried out with ODS column (5 μm, 250 mm×4 6 mm), using methanol water (55∶45) as the mobile plase at a flow rate of 1 mL/min and detected at the wavelength 203 nm Results The recovery of this method was 97.00%, and RSD was 1 60% (n=5) Conclusion The method was simple and accurate and could be used to determine notoginsenoside R1 in JINSENNAOTAI POWDER INJECTION
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[基金项目]
国家新药基金项目(96-901-05-105)